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DEPtech 3DEP Reader

A Dielectrophoresis Cell Anaylsis System

The 3DEP system

uses a patented chip design which is low cost, simple to use and allows the analysis of thousands of cells simultaneously.

Economical

Low cost disposable chips suitable for a large range of cell types from large myocytes to bacteria.

Versatile

Applications include cancer diagnostics, monitoring apoptosis, stem-cell differentiation, drug discovery, in-vitro toxicology and many more.

Publications

Cell analysis papers using the 3DEP system

Dielectric fingerprinting: a low cost, rapid, non-invasive detection tool for oral and oropharyngeal cancer

KA Graham, HJ Mulhall, MP Hughes, MP Lewis, KF Hoettges, N Kalavrezos, J McCaul, C Liew, S Porter, S Fedele, FH Labeed

Cancer Epidemiology, Biomarkers and Prevention  (In press)

 

Human oral cancer cells with increasing tumorigenic abilities exhibit higher effective membrane capacitance

X Liang, KA Graham, AC Johannessen, DE Costea, FH Labeed

Integrative Biology 2014 DOI: 10.1039/c3ib40255j

 

Epithelial cancer cells exhibit different electrical properties when cultured in 2D and 3D environments

HJ Mulhall, MP Hughes, B Kazmi, MP Lewis, FH Labeed

Biochimica et Biophysica Acta 2013 1830 5136-5141

 

Characterisation of human skeletal stem and bone cell populations using dielectrophoresis

A Ismail, MP Hughes, HJ Mulhall, ROC Oreffo, FH Labeed

Journal of Tissue Engineering and Regenerative Medicine 2012 DOI 10-1002/term.1629

 

Cytoplasm resistivity of mammalian atrial myocardium determined by dielectrophoresis and impedance methods

A Manazza, S Salvage, CH Fry, F Labeed, M Hughes, RI Jabr

Biophysical Journal 2012 103 2287-2294

 

Cancer, pre-cancer and normal oral cells distinguished by dielectrophoresis

HJ Mulhall, FH Labeed, B Kazmi, DE Costea, MP Hughes, MP Lewis

Analytical and Bioanalytical Chemistry 2011 401 2455-2463

 

Rapid, automated measurement of dielectrophoretic forces using DEP-activated microwells

LM Broche, KF Hoettges, SL Ogin, GEN Kass, MP Hughes

Electrophoresis 2011 32 2393-2399

 

Biophysical characteristics reveal neural stem cell differentiation potential

FH Labeed, JT Lu, HJ Mulhall, SA Marachenko, KF Hoettges,  LC Estrada, AP Lee, MP Hughes, L FLanagan

PLoS One 2011 6e25458

 

Rapid determination of antibiotic resistance in E. coli using dielectrophoresis

  1. F. Hoettges, J. W. Dale, M. P. Hughes

Physics in Medicine and Biology 2007 52 6001-6009

 

Papers describing 3DEP cell analysis technology

Real-time cell electrophysiology using a multi-channel dielectrophoretic-dot microelectrode array

HO Fatoyinbo, NA Kadri DH Gould, KF Hoettges, FH Labeed

Electrophoresis 2011 32 2541-2549

 

Dielectrophoresis-activated Multiwell Plate for Label-Free High-Throughput Drug Assessment

KF Hoettges Y Hübner, LM Broche, SL Ogin, GEN Kass, MP Hughes

Analytical Chemistry 2008 80 2063-2068

 

Parallel measurements of drug actions on Erythrocytes by dielectrophoresis, using a novel 3-dimensional electrode design

Y Hübner, KF Hoettges, GEN Kass, SL Ogin, MP Hughes

IEE Proceedings on Bionanotechnology 2005 4 21-25

 

Papers on 3DEP separation

Efficient dielectrophoretic cell enrichment using a DEP-well based system

MA Abdul Razak, KF Hoettges, HO Fatoyinbo, FH Labeed, MP Hughes

Biomicrofluidics 2013 7, 064110 (1-10)

 

A 3D dielectrophoretic separator

HO Fatoyinbo, D Kamchis, R Whattingham, SL Ogin, MP Hughes

IEEE Transactions on Biomedical Engineering 2005 52 1347-1349

 

Papers using earlier DEP technology for cell biology

Dielectrophoretic analysis of changes in cytoplasmic ion levels due to ion channel blocker action reveals underlying differences between drug-sensitive and multidrug-resistance leukaemic cells. L Duncan, H Shelmerdine, MP Hughes, HM Coley, Y Hübner, FH Labeed. Physics in Medicine and Biology 2008 53 N1-N7

Early detection of oral cancer – is dielectrophoresis the answer? LM Broche, MP Hughes, M Lewis, WB Barrett, FH Labeed. Oral Oncology 2007 43 199-203

Biophysical characterization of MDR breast cancer cell lines reveals the cytoplasm is critical in determining drug sensitivity. H Coley, FH Labeed, H Thomas, MP Hughes. Biochimica et Biophysica Acta 2007 1770 601-608

Changes in the cytoplasm of K562 myelogeneous leukaemic cancer cells during apoptosis, observed in real time by dielectrophoresis. FH Labeed, HM Coley, H Thomas, MP Hughes. Biophysica et Biochimica Acta 2006 1760 922-929

Rapid detection of early apoptosis events by dielectrophoresis. S Chin, LM Broche, HM Coley, H Thomas, MP Hughes, FH Labeed. International Journal of Nanomedicine 2006 1 333-337

Extraction of dielectric properties for multiple populations from dielectrophoretic collection spectrum data. L Broche, FH Labeed, MP Hughes. Physics in Medicine and Biology 2005 50 2267-2274

Water quality test based on dielectrophoretic measurements of fresh water algae Selenastrum capricornutum. Y Hübner, KF Hoettges, MP Hughes. Journal of Environmental Monitoring 2003 5 861-864

Dielectrophoretic assay of bacterial resistance to antibiotics. J Johari, Y Hübner, J Hull, JW Dale, MP Hughes. Physics in Medicine and Biology 2003 48 N193-N196

Assessment of multidrug resistance reversal using dielectrophoresis and flow cytometry. FH Labeed, HM Coley, H Thomas, MP Hughes. Biophysical Journal 2003 85 2028-2034